Mg2+-activated double-stranded DNA cleavage by [(bpy)2(OH2)RuIIIORuIII(H2O) (bpy)2]4+

Autor:	Suzanne A. Ciftan, Neena Grover, H. Holden Thorp
Rok vydání:	1995
Předmět:	Gel electrophoresis Stereochemistry Chemistry Dimer Photochemistry Cleavage (embryo) humanities Inorganic Chemistry chemistry.chemical_compound Monomer Materials Chemistry DNA supercoil Physical and Theoretical Chemistry Cyclic voltammetry Bond cleavage DNA
Zdroj:	Inorganica Chimica Acta. 240:335-338
ISSN:	0020-1693
DOI:	10.1016/0020-1693(95)04550-3
Popis:	The reaction of the title complex with DNA has been examined. Addition of [(bpy)2(OH2)RuORu(OH2) (bpy)2]4+ to DNA leads to the reduction of the complex to Ru(bpy)2(OH2)22+, as indicated by absorption spectroscopy and cyclic voltammetry. The reaction is accelerated by Mg2+. The combined evidence points to a mechanism where the oxo-bridged dimer is hydrolyzed to a monomeric Ru(III) complex that is capable of oxidizing DNA to effect strand scission. Gel electrophoresis demonstrates nicking of supercoiled /gfX174 DNA by [(bpy)2(OH2)RuORu(OH2) (bpy)2]4+, and double-stranded cleavage is observed in the presence of Mg2+. Linearization of the plasmid prior to treatment with the complex does not lead to further fragmentation, suggesting that supercoiling is required to realize double-stranded cleavage.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9a05fbb474eaf66bf01ab22d2c78d49c https://doi.org/10.1016/0020-1693(95)04550-3 Zobrazit plný text záznamu Full Text from ScienceDirect