Detection of Soil Organic Nitrogen in Xylem Sap Collected from Nonmycorrhizal Plants using an Immunological Technique

Autor:	Junko Kasuga, Mani Rajkumar, Shingo Matsumoto, Taiichiro Yoshida, Noriharu Ae, Jung-Hyen Park
Rok vydání:	2012
Předmět:	Spinacia biology Epidermis (botany) food and beverages Soil Science chemistry.chemical_element Xylem Phosphate biology.organism_classification Nitrogen Oxalate chemistry.chemical_compound chemistry Botany Brassica rapa Spinach Agronomy and Crop Science
Zdroj:	Communications in Soil Science and Plant Analysis. 43:2669-2678
ISSN:	1532-2416 0010-3624
Popis:	Phosphate buffer–extractable organic nitrogen (PEON) is considered to be a ubiquitous high-molecular-weightnitrogen (N) compound in soil and may be an important N source for some plant species. We examined whether nonmycorrhizal plants qing-geng-cai (Brassica rapa var. chinensis) and spinach (Spinacia oleracea L. cv. Atras) could uptake PEON from the soilusing animmunoassay that was previously developed from a PEON determination method using anti-PEON IgG. Xylem sap analysis of spinach using an enzyme-linked immune sorbent assay (ELISA) showed that this plant hadhigh capabilities to take up PEON from the soil. Spinach and qing-geng-cai exuded substantial amounts of oxalate or citrate under inorganic N-deficient conditions, indicating exudation of organic acids is probably involved in PEON mobilization. Further results of immunohistochemical localization of PEON in the root section of qing-geng-cai revealed that intact or at least a fraction of PEON absorbed by the epidermis passes through the cortex cell ...
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::99fb15e91ec2aa2be1b8a24395d717d4 https://doi.org/10.1080/00103624.2012.719249 Zobrazit plný text záznamu