Tissue Factor Pathway Inhibitor-2 Silencing Promotes Hepatocellular Carcinoma Cell InvasionIn Vitro
| Autor: | Guang-Wen Zhang, Zhen Huang, Ping Zeng, Ping Zhang, Ting-Fang Dong, Yong-Kun Gui, Bin Zhu |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
education.field_of_study
Histology biology CD44 Molecular biology Tissue-factor-pathway inhibitor 2 Tissue factor Downregulation and upregulation Cell culture Gene expression Cancer research biology.protein Gene silencing Anatomy education Cell adhesion Ecology Evolution Behavior and Systematics Biotechnology |
| Zdroj: | The Anatomical Record. 296:1708-1716 |
| ISSN: | 1932-8486 |
| DOI: | 10.1002/ar.22789 |
| Popis: | Hepatocellular carcinoma (HCC) is a leading cause of cancer-related death in the world and metastasis is an essential aspect of HCC progression. Tissue factor pathway inhibitor-2 (TFPI-2) has been implicated as a potential suppressor gene to regulate tumor invasion and metastasis. In this study, we silenced TFPI-2 in the HCC cell line MHCC97-L and evaluated the role of TFPI-2 in cell invasion and its impact on gene expression. We showed in this study that stable TFPI-2 downregulation in MHCC97-L cells resulted in increased cell adhesion and invasion. We also showed that mRNA and protein expression levels of MMP-1/3, CD44, and ICAM-1 were increased, while those of MMP-2/9 were not changed by TFPI-2 silencing. Furthermore, silencing of TFPI-2 caused increased Akt phosphorylation level and NF-κB transcription in MHCC97-L cells. In conclusion, this study confirms that TFPI-2 downregulation can contribute to tumor invasion of HCC cells through alteration in the expression of metastasis-related genes. |
| Databáze: | OpenAIRE |
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