Evaluation of Penicillium chrysogenum and its antifungal extracts as potential biological control agents against Botrytis fabae on faba beans
| Autor: | A. J. Jackson, D. R. Walters, G. Marshall |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
biology
fungi Ethyl acetate food and beverages Plant Science biology.organism_classification Penicillium chrysogenum Spore chemistry.chemical_compound chemistry Germination Botany Genetics Spore germination Botrytis fabae Petroleum ether Food science Ecology Evolution Behavior and Systematics Mycelium Biotechnology |
| Zdroj: | Mycological Research. 98:1117-1126 |
| ISSN: | 0953-7562 |
| DOI: | 10.1016/s0953-7562(09)80197-4 |
| Popis: | Antagonistic fungal isolates (AP1.S20, AP2.R16, Z1.S23 and AP2.R19), from root and soil samples of faba bean crops, inhibited mycelial growth of Botrytis fabae in vitro by up to 44·9%, producing clear inhibition zones. Spore suspensions of isolates AP2.R16, Z1.S23 and especially AP1.S20, significantly reduced the development of chocolate spot lesions on bean plants in the glasshouse. These isolates were identified as Penicillium chrysogenum. The cell free culture filtrate of AP1.S20 was screened in vitro for antifungal activity using four solvent extracts: ethyl acetate, chloroform, hexane and petroleum ether. The ethyl acetate fraction gave the largest inhibition of spore germination (95·7%), while the petroleum ether fraction significantly reduced germ-tube extension to 19% of the control. Thin layer chromatography of these solvent extracts displayed up to 15 discrete fractions from each solvent sample. On application of these to tlc plates, the germination of B. fabae conidia was inhibited at one discrete spot from each solvent extract, each with the same Rf value. Preliminary results using detached leaves showed that both the culture filtrate and a spore suspension of isolate AP1.S20 significantly reduced lesion development and lesion spread. |
| Databáze: | OpenAIRE |
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