Protective Effects Of A Lipid Transfer Protein Isolated from Morinda citrifolia Seeds in Gentamicin-Induced Nephrotoxicity in Rats
| Autor: | Luana David do Carmo, Hermógenes David de Oliveira, Alice Maria Costa Martins, Francisco Assis Nogueira-Junior, Pedro Luiz Lopes, Roberta Jeane Bezerra Jorge, Tiago Lima Sampaio, Francisco A. F. Xavier-Junior, Helena Serra Azul Monteiro, Aline Diogo Marinho, João Alison de Moraes Silveira, Janaina Serra Azul Monteiro Evangelista, Adriano José Maia Chaves-Filho, Dyély de Carvalho Oliveira Campos, Nylane M.N. Alencar, Danielle Silveira Macêdo, Pedro Henrique Sá Costa, Natacha Teresa Queiroz Alves |
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| Rok vydání: | 2020 |
| Předmět: |
biology
010405 organic chemistry medicine.medical_treatment Intraperitoneal injection Aminoglycoside Pharmacology biology.organism_classification medicine.disease_cause 01 natural sciences 0104 chemical sciences Nephrotoxicity 010404 medicinal & biomolecular chemistry chemistry.chemical_compound Morinda chemistry In vivo medicine Uric acid Gentamicin General Pharmacology Toxicology and Pharmaceutics Oxidative stress medicine.drug |
| Zdroj: | Revista Brasileira de Farmacognosia. 30:568-576 |
| ISSN: | 1981-528X |
| DOI: | 10.1007/s43450-020-00087-5 |
| Popis: | Gentamicin is an aminoglycoside antibiotic used in the treatment of gram-negative bacterial infections. However, its prolonged use is limited due to a high risk of nephrotoxicity. This study aimed to investigate the protective effects of a lipid transfer protein isolated from Morinda citrifolia L., Rubiaceae, seeds against gentamicin-induced nephrotoxicity in rats, and in a proximal tubular epithelial cell line from Rhesus monkey (LLC-MK2). Treatment with 6.25–25 μg/ml of the protein protected cells against cytotoxicity induced by 2 mM gentamicin. In the in vivo study, protein treatment (8 mg/kg, orally administered daily for 7 days 2 h after intraperitoneal injection of 100 mg/kg gentamicin) improved the levels of creatinine, urea, and uric acid. Additionally, it alleviated oxidative stress and inflammation induced by gentamicin, including a reduction in myeloperoxidase activity and cytokine modulation. Data were corroborated with histological analysis showing attenuated alterations by protein treatment. These findings suggest that this protein protects against gentamicin-induced nephrotoxicity. In addition, this natural product may be as an adjunctive tool to reduce kidney toxicity and improve the clinical usage profile of gentamicin. |
| Databáze: | OpenAIRE |
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