Enhancement of Kraft lignin molecular relaxation based on laccases from Pycnoporus sanguineus produced in instrumented bioreactors
| Autor: | Nei Pereira, Verônica Calado, Felipe Souto, Evanildo F. Souza, Douglas de França Passos |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Laccase
biology Renewable Energy Sustainability and the Environment Chemistry fungi technology industry and agriculture food and beverages Continuous stirred-tank reactor macromolecular substances biology.organism_classification complex mixtures chemistry.chemical_compound Chemical engineering Bioreactor Lignin Fiber Pycnoporus sanguineus Homogenization (biology) Bubble column reactor |
| Zdroj: | Biomass Conversion and Biorefinery. |
| ISSN: | 2190-6823 2190-6815 |
| Popis: | This study has been focused on obtaining fungal laccases and structural modification of lignin by enzymatic action in laccase-ABTS system, mainly reducing molecular stiffness. The best specific aeration rate in bubble column reactor is 0.67 vvm, with 3260 U/L laccase activity, and enzymatic activity increased by 60% in stirred tank reactor. Enzymatic cocktail has been concentrated in a hollow fiber system and partially characterized. Laccase-ABTS system promoted significant thermal and structural changes in Kraft lignin in all essayed reaction times (2, 24, 48, and 72 h). The best reaction time of the enzymatic treatment is LT-lac48, which promoted the highest relaxation coefficient (18.0%). The enzymatic cocktail produced herein is efficient for structural modification of Kraft lignin. The positive evidence of lignin’s relaxation/flexibility and its linearization and homogenization suggests that treatment performed may adjust studied lignin to produce high-performance lignin suitable for many applications. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink