| Autor: |
R. Byrne, Hans P. Kiener, B. E. M. Bachmann, Wolfgang Holnthoner, I Olmos Calvo, J Holinka, S. Schobesberger, Ariane Fischer, Peter Ertl, Reinhard Windhager, Stefan Toegel, Sarah Spitz, Heinz Redl, Florian Sevelda, Mario Rothbauer, E. Reihs |
| Rok vydání: |
2021 |
| Předmět: |
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| DOI: |
10.1101/2021.02.19.431936 |
| Popis: |
Rheumatoid arthritis is characterised by a progressive, intermittent inflammation at the synovial membrane, which ultimately leads to the destruction of the synovial joint. The synovial membrane, which is the joint capsule’s inner layer, is lined with fibroblast-like synoviocytes that are the key player supporting persistent arthritis leading to bone erosion and cartilage destruction. While microfluidic models that model molecular aspects of bone erosion between bone-derived cells and synoviocytes have been established, RA’s synovial-chondral axis has yet not been realised using a microfluidic 3D model based on human patient in vitro cultures. Consequently, we established a chip-based three-dimensional tissue co-culture model that simulates the reciprocal cross-talk between individual synovial and chondral organoids. We now demonstrate that chondral organoids, when co-cultivated with synovial organoids, induce a higher degree of cartilage physiology and architecture and show differential cytokine response compared to their respective monocultures highlighting the importance of reciprocal tissue-level cross-talk in the modelling of arthritic diseases. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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