Cytological characteristics of microconidia of Magnaporthe oryzae

Autor:	Naoki Hosogi, Izumi Chuma, Takeshi Shinogi, Kenichi Ikeda, Pyoyun Park, Yukio Tosa, Hitoshi Nakayashiki
Rok vydání:	2009
Předmět:	Hypha fungi Phialide Germ tube RNA Plant Science Biology Green fluorescent protein Staining Microbiology Conidium Cell biology medicine.anatomical_structure medicine Agronomy and Crop Science Nucleus
Zdroj:	Journal of General Plant Pathology. 75:353-358
ISSN:	1610-739X 1345-2630
DOI:	10.1007/s10327-009-0181-1
Popis:	The inner cellular structure of microconidia of Magnaporthe oryzae was examined using fluorescent probes and electron microscopic techniques. The volume of the nucleus relative to the cell was significantly larger in microconidia than in macroconidia or vegetative hyphae, similar to observations for spermatia of other fungi. Selective fluorescent staining revealed that cytosolic RNA was less abundant in microconidia than in macroconidia and germ tubes, suggesting that general metabolic activity in microconidia is low. Consistently, GFP expression driven by the TrpC promoter was highly active during the formation of phialides and microconidia but gradually decreased as the microconidia matured. Such data suggest that microconidia are in a quiescent or dormant state.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::999fcf352d333a7973e94b3b756329ad https://doi.org/10.1007/s10327-009-0181-1 Zobrazit plný text záznamu Full text from SpringerLink