| Popis: |
The conventional orange peel extraction processes have several drawbacks hence; there is a need to develop green process for extraction of orange oil. In present study, solid state fermentation of fresh orange peels by Aspergillus species NCIM -1432 to extract extra-cellular enzymes and orange peel oil are noted and the effect of methods of orange peel sterilisation, importance of selection of the fermentation strain, external nitrogen source, and particle size of the peels are also studied. The process used here gave 0.95 % w/w yield of orange oil with 96 % w/w limonene content with typical orange colour and flavour. Also, the crude enzymes produced during fermentation showed 155 U/ g and 239 CMC/ g respectively of pectinase and cellulase activity. The molecular weights of the obtained enzymes were found to be 39, 55 and 120 kDa which corresponds to the reported pectinase and cellulase. |
