Variant Hemoglobin Spectrum By Cation Exchange High Performance Liquid Chromatography: A Study 0f 2035 Subjects
| Autor: | Ankur N Sarvaiya, Sanjaykumar C. Chauhan |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
medicine.medical_specialty
Sickle cell trait Heterozygous Hb-S business.industry Thalassemia medicine.disease BETA THALASSEMIA MAJOR High-performance liquid chromatography Gastroenterology Abnormal hemoglobin Labor saving hemic and lymphatic diseases Internal medicine medicine General Earth and Planetary Sciences Hemoglobin business General Environmental Science |
| Zdroj: | Annals of Pathology and Laboratory Medicine. 4:A297-A302 |
| ISSN: | 2349-6983 2394-6466 |
| DOI: | 10.21276/apalm.1393 |
| Popis: | Introduction Hemoglobinopathies and thalassemia are hereditary disorders of hemoglobin (Hb) affecting mankind at prevalent regional level. Automated cation exchange high performance liquid chromatography is being increasingly used as the initial diagnostic method for identifying normal and abnormal hemoglobin variants. Methodology Total 2035 sample received and studied. All samples run on cation exchange high performance liquid chromatography machine by BIO-RAD. Results: Total 386(18.96%) cases had abnormal hemoglobin fractions. Beta thalassemia major cases were 17(0.83%). Beta thalassemia intermedia cases were 4(0.19%). Sickle cell trait (heterozygous Hb S) cases were 96(4.71%). Double heterozygous for sickle cell-beta thalassemia cases were 14(0.68%). There were 06 cases (0.29%) of Hb D heterozygous. There were one each case of Hb E heterozygous and Hb E-beta thalassemia (0.04% each). Hb F was raised in 7 adult cases (0.34%). Conclusion: To conclude, cation exchange high performance liquid chromatography is less time consuming, cost effective, labor saving, reproducible, accurate, sensitive and specific method to detect hemoglobinopathies and thalassemia. Most of the abnormal cases are diagnosed with this method, with few inconclusive cases require further genetic and molecular workup. DOI: 10.21276/APALM.1393 |
| Databáze: | OpenAIRE |
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