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The main objective of this study is to analyze the salted fish to determine the concentrations of biogenic amines in relation to their sodium chloride concentrations. Forty random samples of salted Sardine and Mugil cephalus fish” Feseekh” (20 of each) were collected from different fish markets in El- Gharbia and Kafr El-shiekh Governorates, Egypt. The collected samples were packed in polyethylene bags and stored at -20oC till be analyzed for determination of their sodium chloride concentrations by titration method and biogenic amines using High Performance Liquid Chromatography(HPLC). The obtained results revealed that the mean sodium chloride concentrations were 12.72 ± 4.02 and 14.20 ± 3.18 for salted Sardine and Feseekh, respectively. On the other hand the mean concentration of histamine was 19.26± 11.83 and 16.02±10.54 mg/ 100g of the examined salted sardine and feseekh samples, respectively .While the mean concentration of tyramine was 13.03± 8.55 and 11.21± 7.28, respectively for the same examined samples. Concerning to cadeverine the mean concentration was 10.09± 6.04 and 7.84± 5.64, respectively. Comparing the obtained results with the permissible limit (20mg/100g) recommended by FDA (2001), 35, 20 and 10% of the examined salted Sardine samples exceeded the limit for histamine, tyramine and cadaverine, while 30, 20 and 5% of the examined feseekh samples exceeded the permissible limit for histamine, tyramine and cadaverine, respectively. This study show that there is a Significant negative correlation between sodium chloride percent and biogenic amines concentration in the examined salted fish samples for histamine While there is unsignificant negative correlation between sodium chloride percent and cadaverine concentration in the examined salted fish samples |
