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The present study focused on biosynthesis of silver nanoparticles from an endophytic fungus isolated from the medicinal plant Abrus precatorius. The endophytic fungus was identified as Phyllosticta owaniana KUMBMDBT-32 (NCBI Accession number MW007919). The fungus was cultured in submerged fermentation and extracted with ethyl acetate (1:1 V/V). The extract was subjected to GC-MS analysis which have revealed the presence of 42 bioactive compounds of secondary metabolites. Synthesized silver nanoparticles were characterized by Bio-spectrophotometer, FTIR, SEM-EDX, XRD and DLS. The absorption spectra in the Bio-spectrophotometer analysis showed a peak at 400 nm, confirmed the synthesis of silver nanoparticles. The FTIR study revealed peaks corresponding to various functional groups possibly involved, confirming the reducing and capping of silver nanoparticles. The spherical form of silver nanoparticles was revealed through SEM-EDAX studies, the EDAX study showed that silver atoms are present at 3keV. XRD studies revealed the crystalline structure of the silver nanoparticles. DLS study determined size of synthesized silver nanoparticles i.e., 65.81 nm. Antibacterial activity of synthesized silver nanoparticles was tested against pathogenic bacteria. S. aureus, E. coli, P. aeruginosa, S. typhi and K. pneumoniae. Antibiotic enhancing activity of silver nanoparticles was determined by using commercial antibiotics such as Chloramphenicol, Cefpodoxime, Gentamicin, Ampicillin, and Imipenem. Antifungal activity silver nanoparticles was determined against fungal pathogens such as Candida albicans, Aspergillus brassiliensis, and Aspergillus flavus. Synthesized silver nanoparticles exhibited effective antioxidant, measurement of cell viability by MTT assay and in-vitro and in-vivo anti-inflammatory activities. These results certainly determines pharmaceutical and biomedical importance of silver nanoparticles. |
