Model to study in vivo transdifferentiation of somatic cells into urothelium

Autor: N. A. Nitsa, Boris Popov, N. S. Petrov, A. M. Zaichik, M. B. Budko, B. K. Komyakov, O. V. Zhidkova, S. A. Koshkin, E. N. Tolkunova
Rok vydání: 2010
Předmět:
Zdroj: Cell and Tissue Biology. 4:511-519
ISSN: 1990-5203
1990-519X
Popis: The development of reconstructive therapy of the urinary tract using pluripotent and somatic stem cells, e.g., mesenchymal stem cells (MSC), is currently in the stage of experimental studies. These studies include the investigation of the main functions of MSC and the urothelium lining the organs of the urinary tract. An important role in the regulation of proliferation and differentiation of urothelium belongs to EGF and Wnt/β-catenin signaling pathways, the activity of which may be evaluated by the level of Her-4 and Tcf-3, 4, respectively. We found that MSC labeled by transgenic green fluorescence protein (GFP) did not pro-duce Her-4 and Tcf3, 4 in vitro, but activated their production after cell grafting into the cryoinjured bladders of the syngenic mice. In mice transplanted with these MSC GFP was detected by RT-PCR in the bladder. GFP colocalization with Her-4 or Tcf3, 4 in a few urothelial cells was detected by immunohistochemical staining with specific antibodies. These results suggest that MSC labeled with GFP an be used as a proper model to study the transdifferentiation of somatic cells into urothelium.
Databáze: OpenAIRE
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