Study of the Partitioning of Red Blood Cells Through Asymmetric Bifurcating Microchannels
| Autor: | Hiroki Abe, Ko Yamamoto, Haruyuki Ogura, Chihiro Miyoshi, Toru Hyakutake |
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| Rok vydání: | 2019 |
| Předmět: |
Materials science
Physics::Medical Physics 0206 medical engineering Flow (psychology) Biomedical Engineering 02 engineering and technology General Medicine Mechanics Division (mathematics) Velocimetry 020601 biomedical engineering Quantitative Biology::Cell Behavior 030218 nuclear medicine & medical imaging Volumetric flow rate Physics::Fluid Dynamics 03 medical and health sciences 0302 clinical medicine Microfluidic channel Turning point Axial symmetry Bifurcation |
| Zdroj: | Journal of Medical and Biological Engineering. 40:53-61 |
| ISSN: | 2199-4757 1609-0985 |
| Popis: | The heterogeneous distribution of red blood cells (RBCs) in the microcirculatory system is a complicated phenomenon because many factors influence how they are partitioned at microvascular bifurcations. In this study, we investigated the RBC partitioning mechanism by fabricating an in vitro experimental apparatus. The apparatus comprised a bifurcating, microfluidic channel, and the flow rate through the channels can be controlled readily over a wide range. The division turning point at which an RBC trajectory changes from one bifurcation to the other was identified via particle-tracking velocimetry on individual RBCs. The experimental results were close to the theoretical predictions and numerical simulations. The hematocrit variation before and after bifurcation was quantitatively estimated by image analysis and compared to the prediction based on Pries’ empirical model. The Zweifach–Fung effect was enhanced in the smaller fractional flow, increasing the RBC flux bias. Moreover, a cell-free layer with axial symmetry was formed in the parent channel, and an asymmetric cell-free layer was formed immediately after bifurcation. These obtained results will help clarify the RBC partitioning mechanism at microvascular bifurcations. |
| Databáze: | OpenAIRE |
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