Factors regulating bone remodeling processes in aseptic implant loosening
| Autor: | Felicitas Huber, Burkhard Summer, Julia I. Redeker, Andreas Fottner, Marcus Schmitt-Sody, Baerbel Schmitt, Eliza S. Hartmann, Susanne Mayer-Wagner, Volkmar Jansson, Miriam I. Köhler |
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| Rok vydání: | 2016 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine Cathepsin 030222 orthopedics biology Chemistry Proteolytic enzymes Histology Bone remodeling Andrology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine medicine.anatomical_structure Osteoclast RANKL medicine biology.protein Cathepsin K Immunohistochemistry Orthopedics and Sports Medicine |
| Zdroj: | Journal of Orthopaedic Research. 35:248-257 |
| ISSN: | 0736-0266 |
| DOI: | 10.1002/jor.23274 |
| Popis: | This study was undertaken to screen periprosthetic tissues (PPTs) under specified conditions for a series of molecular components and describe them in bone remodeling processes within aseptic loosening. PPT samples were obtained from patients undergoing revision surgery of endoprostheses (n = 24) and synovial tissues from patients with OA (control) (n = 18), patients with any form of inflammatory arthritides were excluded. Tissue samples were examined via microbiology, histology (H&E, TRAP), immunohistochemistry (CD68/anti-S100a4), quantitative real-time PCR (ALP, COL1A1, cathepsin K, M-CSF, MMP13, OPG, RANK, RANKL, TNF-α, and TRAP) and an endotoxin-assay. PPT samples contained a variety of cellular components and stained positive for TRAP (56%), CD68 (100%), and S100a4 (100%). Wear debris were found in cells staining positive for CD68 and S100a4. In PPTs significantly higher ALP, COL1A1, MMP-13, RANK, RANKL, and TRAP expression were found along with a significantly higher RANKL/OPG ratio and a significantly lower OPG expression. No significant difference was observed for M-CSF, TNF-α, cathepsin K, and endotoxin levels. In conclusion we found osteogenic proteins (ALP, COL1A1), a proteolytic enzyme (MMP-13), markers for osteoclast differentiation (RANK, RANKL), and osteoclast activity (TRAP) to be increased in PPT, whereas OPG expression decreased significantly in comparison to control. We present data about a large series of molecular components in PPT and describe novel and key findings about their expression levels in regards to aseptic implant loosening. © 2016 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 35:248-257, 2017. |
| Databáze: | OpenAIRE |
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