| Autor: | K.C. Giles, William J. Smith, Clark L. Gross |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
medicine.diagnostic_test
Health Toxicology and Mutagenesis medicine.medical_treatment Lymphocyte Sulfur mustard Cell Biology Glutathione Tripeptide Pharmacology Toxicology Flow cytometry chemistry.chemical_compound medicine.anatomical_structure chemistry Biochemistry medicine Propidium iodide Antidote Cytotoxicity |
| Zdroj: | Cell Biology and Toxicology. 13:167-173 |
| ISSN: | 0742-2091 |
| DOI: | 10.1023/a:1007354127919 |
| Popis: | Sulfur mustard (HD, mustard gas) is a vesicant chemical warfare agent for which there is no specific medical countermeasure. A potential approach to combating the debilitating effects of this agent is the use of compounds that can react with this material before it interacts with critical macro-molecules. Glutathione (GSH), a tripeptide that exists in high concentrations in cells, reacts with HD and is involved in HD detoxification. Pretreatment of human peripheral blood lymphocytes (PBL) with 10 mmol/L L-oxothiazolidine-4-carboxylate (OTC), a "masked" cysteine precursor, increased intracellular glutathione levels 25-50% over control values. Pretreated PBL were harvested, washed, and exposed to 10, 50, or 100 µmol/L HD. Flow cytometry was used to measure cytotoxicity by propidium iodide uptake. Pretreatment of PBL with OTC led to small decreases in cytotoxicity after HD exposure. However, treatment of cells with OTC after HD exposure was not beneficial. Compounds that can modulate GSH levels within the cell may help to reduce the cytotoxicity of HD when used as a pretreatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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