| Autor: |
Ann F. Hanham, Michael J. Smith |
| Rok vydání: |
1980 |
| Předmět: |
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| Zdroj: |
Comparative Biochemistry and Physiology Part B: Comparative Biochemistry. 65:333-338 |
| ISSN: |
0305-0491 |
| DOI: |
10.1016/0305-0491(80)90022-x |
| Popis: |
1. 1. The hydroxyapatite reassociation kinetics of coho and sockeye DNA at a fragment length of 450 bp have been determined and compared with chum salmon. 2. 2. All three species have approx 60% of the genomic DNA in reiterated sequences with repetition frequencies that range from tens to thousands of times per genome. 3. 3. Single-copy DNA at a fragment length of 275 bp was prepared from chum salmon and labeled with 125 I. 4. 4. 93% of the reactable [ 125 I]DNA hybridized with an excess of coho DNA with second-order reaction kinetics. 5. 5. 73% of the reactable chum single-copy [ 125 I]DNA hybridized with an excess of sockeye DNA. 6. 6. The denaturation temperature of chum single-copy [ 125 I]DNA hybridized with either coho or sockeye DNA was depressed 2°C from that of the homologous duplex. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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