A twinfilin-like protein coordinates karyokinesis by influencing mitotic spindle elongation and DNA replication inLeishmania
| Autor: | Lova Prasadareddy Kajuluri, Chhitar M. Gupta, Gaurav Kumar, Amogh A. Sahasrabuddhe |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Genetics Mitotic spindle elongation 030102 biochemistry & molecular biology Nucleolus DNA replication Biology Microbiology Spindle elongation Spindle pole body Cell biology Spindle apparatus 03 medical and health sciences 030104 developmental biology Basal body Molecular Biology Mitosis |
| Zdroj: | Molecular Microbiology. 100:173-187 |
| ISSN: | 0950-382X |
| Popis: | Twinfilin is an evolutionarily conserved actin-binding protein, which regulates actin-dynamics in eukaryotic cells. Homologs of this protein have been detected in the genome of various protozoan parasites causing diseases in human. However, very little is known about their core functions in these organisms. We show here that a twinfilin homolog in a human pathogen Leishmania, primarily localizes to the nucleolus and, to some extent, also in the basal body region. In the dividing cells, nucleolar twinfilin redistributes to the mitotic spindle and remains there partly associated with the spindle microtubules. We further show that approximately 50% depletion of this protein significantly retards the cell growth due to sluggish progression of S phase of the cell division cycle, owing to the delayed nuclear DNA synthesis. Interestingly, overexpression of this protein results in significantly increased length of the mitotic spindle in the dividing Leishmania cells, whereas, its depletion adversely affects spindle elongation and architecture. Our results indicate that twinfilin controls on one hand, the DNA synthesis and on the other, the mitotic spindle elongation, thus contributing to karyokinesis in Leishmania. |
| Databáze: | OpenAIRE |
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