Iohexol in Serum Determined by Capillary Electrophoresis

Autor:	M S Constantinescu, Z K Shihabi
Rok vydání:	1992
Předmět:	Chromatography Chemistry Biochemistry (medical) Clinical Biochemistry Renal function Serum samples Dilution chemistry.chemical_compound Capillary electrophoresis BORATE BUFFER medicine Iohexol Acetonitrile Quantitative analysis (chemistry) medicine.drug
Zdroj:	Clinical Chemistry. 38:2117-2120
ISSN:	1530-8561 0009-9147
DOI:	10.1093/clinchem/38.10.2117
Popis:	Iohexol, a nonionic compound used as a contrast medium for angiography and as a measure of the glomerular filtration rate, was quantified in serum by capillary electrophoresis. Comparable results were obtained for serum samples deproteinized with acetonitrile or analyzed directly after 50-fold dilution with borate buffer. Serum samples were electrophoresed for 2.6 min at 12 kV in a borate buffer with detection at 254 nm and with 3-isobutyl-1-methylxanthine as internal standard. Acetonitrile deproteinization gave a greater sensitivity than did sample dilution. Between-run CVs were between 4.7% and 6.7%, and within-run CVs were between 2.5 and 3.2%. Analytical recoveries were 95-105%. Results of the method compared well with those by high-performance liquid chromatography (slope 0.96, intercept 0.005 g/L). This method demonstrates the potential of capillary electrophoresis for rapid and simple quantification of small molecules.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9921b9136876f3c1e22864c9517f0f98 https://doi.org/10.1093/clinchem/38.10.2117 Zobrazit plný text záznamu