Efficient miRNA Inhibitor Delivery with Graphene Oxide-Polyethylenimine to Inhibit Oral Squamous Cell Carcinoma
| Autor: | Lingling Ou, Xiaozhen Zhan, Lihong Lu, Longquan Shao, Ye Zhang, Qingtong Zhao, Renfa Lai, Zhiying Zhou, Minyi Liu, Ting Sun |
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| Rok vydání: | 2020 |
| Předmět: |
Genetic enhancement
Biophysics Pharmaceutical Science Bioengineering macromolecular substances 02 engineering and technology 010402 general chemistry 01 natural sciences Metastasis Biomaterials chemistry.chemical_compound Drug Discovery microRNA medicine PTEN Polyethylenimine biology Chemistry Organic Chemistry technology industry and agriculture General Medicine Transfection 021001 nanoscience & nanotechnology medicine.disease 0104 chemical sciences stomatognathic diseases Apoptosis biology.protein Cancer research 0210 nano-technology Intracellular |
| Zdroj: | International Journal of Nanomedicine. 15:1569-1583 |
| ISSN: | 1178-2013 |
| DOI: | 10.2147/ijn.s220057 |
| Popis: | Background MicroRNAs (miRNAs) are widely believed to be promising targets for oral squamous cell carcinoma (OSCC) gene therapy. miR-214 has been identified as a promoter of OSCC aggression and metastasis. Methods Graphene oxide-polyethylenimine (GO-PEI) complexes were prepared and loaded with a miRNA inhibitor at different N/P ratios. The transfection efficiency of GO-PEI-inhibitor was tested in Cal27 and SCC9 cells. Moreover, the tumor inhibition ability of GO-PEI-inhibitor was measured in an OSCC xenograft mouse model by intratumoral injection. Results Here, we show that a GO-PEI complex efficiently delivers a miR-214 inhibitor into OSCC cells and controls the intracellular release of the miR-214 inhibitor. These results indicate that the GO-PEI-miR-214 inhibitor complex efficiently inhibited cellular miR-214, resulting in a decrease in OSCC cell invasion and migration and an increase in cell apoptosis by targeting PTEN and p53. In the xenograft mouse model, the GO-PEI-miR-214 inhibitor complex significantly prevented tumor volume growth. Conclusion This study indicates that functionalized GO-PEI with low toxicity has promising potential for miRNA delivery for the treatment of OSCC. |
| Databáze: | OpenAIRE |
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