| Popis: |
To control spread of Schistosma mansoni infection, rapid investigation of infected Biomphalaria Alexandrina snails that surveyed from any suspected area of a field, must be evaluated before shedding of cercariae. Detection of S. mansoni antigens in snails hemolymph was exposed to be an accurate and rapid method for assessment of infected snails. In the present study, two murine monoclonal antibodies (MABs) against soluble egg antigen (SEA) were employed for detecting S. mansoni in the hemolymph of 143 infected B. Alexandrina snails and 88 normal control snails, by using an enzyme linked immunosorbent assay (sandwich ELISA). Also, 63 snails and 56 snails of B. glabrata and Bulinus truncularia respectively were examined. The optical density (OD) values of SEA were measured in the hemolymph of infected snails. A total of 143 infected snails showed 100% percentage of positivety. Optical density at intervals 1, 2, 3 weeks post-exposure (prepatency) was measured and percentage positivity was 7%, 35.3%, 82.6%, respectively. It is also measured at early shedding snails (4 weeks, i.e. early patency) and at shedding (5, 6 weeks or maximal shedding) and percentage positivity was 87.5%, 96.2%, 89.5%, respectively. Also, after stop shedding (late patency) the percentage positivity was 15%.The hemolymph of normal control group of snails were all negative against SEA. Hemolymph of B.trunculata and B. glabrata infected snails showed 0% positivity against SEA. Polymorphic DNA (RAPD-PCR) technique was used for molecular identification of the infected snails at the time of 2 weeks post-infection and at the time of snails shedding. Conclusion The use of monoclonal antibodies for early detection of S. mansoni infected B. Alexandrina snails, is considered as an accurate, rapid and specific method to facilitate the control of infected snails that randomly surveyed from different areas of a suspected fields. |
Full Text from ScienceDirect