Measurement of nanosecond time-resolved fluorescence with a directly gated interline CCD camera
| Autor: | J. E. Wall, J. G. Murray, Christopher G. Morgan, A. C. Mitchell |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Pulsed laser
Fluorescence-lifetime imaging microscopy Histology Materials science Ccd camera business.industry ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION Image intensifier Nanosecond Pathology and Forensic Medicine law.invention Optics law Image sensor Time-resolved spectroscopy business Excitation |
| Zdroj: | Journal of Microscopy. 206:233-238 |
| ISSN: | 1365-2818 0022-2720 |
| DOI: | 10.1046/j.1365-2818.2002.01030.x |
| Popis: | CCD cameras coupled optically to gated image intensifiers have been used for fast time-resolved measurements for some years. Image intensifiers have disadvantages, however, and for some applications it would be better if the image sensor could be gated directly at high speed. Control of the 'charge drain' function on an interline-transfer CCD allows the sensor to be switched rapidly from an insensitive state. The temporal and spatial properties of the charge drain are explored in the present paper and it is shown that nanosecond time resolution with acceptable spatial uniformity can be achieved for a small commercial sensor. A fluorescence lifetime imaging system is demonstrated, based on a repetitively pulsed laser excitation source synchronized to the CCD control circuitry via a programmable delay unit. |
| Databáze: | OpenAIRE |
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