Novel technologies applied to the nucleotide sequencing and comparative sequence analysis of the genomes of infectious agents in veterinary medicine
| Autor: | S Belák, F Granberg, Á Bálint |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Comparative genomics Veterinary medicine Massive parallel sequencing Genomics General Medicine Nucleic acid amplification technique Biology Genome DNA sequencing 03 medical and health sciences 030104 developmental biology Metagenomics Infectious disease (medical specialty) Animal Science and Zoology |
| Zdroj: | Revue Scientifique et Technique de l'OIE. 35:15-42 |
| ISSN: | 0253-1933 |
| DOI: | 10.20506/rst.35.1.2415 |
| Popis: | Next-generation sequencing (NGS), also referred to as deep, high-throughput or massively parallel sequencing, is a powerful new tool that can be used for the complex diagnosis and intensive monitoring of infectious disease in veterinary medicine. NGS technologies are also being increasingly used to study the aetiology, genomics, evolution and epidemiology of infectious disease, as well as host-pathogen interactions and other aspects of infection biology. This review briefly summarises recent progress and achievements in this field by first introducing a range of novel techniques and then presenting examples of NGS applications in veterinary infection biology. Various work steps and processes for sampling and sample preparation, sequence analysis and comparative genomics, and improving the accuracy of genomic prediction are discussed, as are bioinformatics requirements. Examples of sequencing-based applications and comparative genomics in veterinary medicine are then provided. This review is based on novel references selected from the literature and on experiences of the World Organisation for Animal Health (OIE) Collaborating Centre for the Biotechnology-based Diagnosis of Infectious Diseases in Veterinary Medicine, Uppsala, Sweden. |
| Databáze: | OpenAIRE |
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