Electrochemical biotesting technique as applied to comparative assessment of antimicrobial properties of essential oils
| Autor: | V. S. Sibirtsev, V.L. Kabanov, U. Yu. Nechiporenko, M. Yu. Kukin |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0303 health sciences
Liquid culture medicine.drug_class Chemistry Microorganism 030302 biochemistry & molecular biology Antibiotics Biological activity Raw material Antimicrobial medicine.disease_cause 01 natural sciences 010309 optics 03 medical and health sciences Staphylococcus aureus 0103 physical sciences medicine Food science Visual methods |
| Zdroj: | Toxicological Review. :50-55 |
| ISSN: | 0869-7922 |
| DOI: | 10.36946/0869-7922-2021-29-3-50-55 |
| Popis: | A biotesting technique is described that provides for periodic (every 2 hours) recording of changes in pH, redox potential, and electrical conductivity of a liquid culture medium incubated in the presence and in the absence of viable test microorganisms (TM) and test samples (TS). The results of a comparative analysis using this technique of antibiotic activity against Staphylococcus aureus of different concentrations of «essential oils» obtained from 10 types of plant raw materials are presented. Based on this, we can conclude the following. Using the presented methodology, it is possible to assess the effect on the dynamics of the vital activity of TM of samples of various pharmaceutical, cosmetic, food, feed and other products, much more quickly, objectively and informatively than using standard visual methods of microbiological testing. The initial antibiotic activity of TS in most cases was greater than their prolonged antibiotic activity. At the same time, the mid-term (in terms of the time of interaction of TS with TM) antibiotic activity of TS was usually intermediate in value between their initial and prolonged biological activity. |
| Databáze: | OpenAIRE |
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