| Autor: |
G. A. Palma, Karin Schütze, Annette Clement-Sengewald, H. Poesl, Gottfried Brem, A. Heinze |
| Rok vydání: |
1993 |
| Předmět: |
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| Zdroj: |
Lasers in Otolaryngology, Dermatology, and Tissue Welding. |
| ISSN: |
0277-786X |
| DOI: |
10.1117/12.147030 |
| Popis: |
A UV-laser microbeam was successfully used to induce fusion of early embryonic cells. The developmental capacity of the laser-fused cells was examined using in vitro culture methods. Blastomeres within mouse two-cell embryos were fused with 3 - 10 subsequent laser pulses in order to produce tetraploid embryos. Thirty-one percent of the laser treated embryos fused and 10% of those developed to the morula or blastocyst stage. With 1 - 10 successive laser pulses cattle oocytes were fused with cytoplasts. Thirty-six percent of the laser treated cells fused and 10% of those cleaved to the 6- and 8-cell stage. These preliminary results indicate that a UV- laser microbeam combined with an optical tweezers may facilitate the manipulation of embryonic cells and can be a helpful tool in polyploidy studies and in cytoplasmic transfer experiments. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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