Effects of enhancing p21 waf1 on proliferation and expression of G1cyclins andCDKs in human breast cancer cells

Autor: Ying-jiang Ye, Yong-chao Wang, Jian-li Sang
Rok vydání: 1999
Předmět:
Zdroj: Chinese Science Bulletin. 44:1489-1493
ISSN: 1861-9541
1001-6538
DOI: 10.1007/bf03183569
Popis: The cyclin-dependent kinase inhibitor p21(waf1/cip1/sdi1) is an important negative regulator in control of cell cycle. Its functions of inhibiting cancer cell growth and its effects on expression of G1 phasecyclins and relatedCDKs are a worthy topic for study. The plasmid expressing p21 with high level was transformed to human breast cancer cells, and the expression of p21 in cells was enhanced, then the cell growth rate, anchorage-independent growth and tumorigenecity were tested, at the same time the expression levels ofcyclinD1, CDK4, cyclinE andCDK2 were analyzed by Northern blot. The results showed that since the expression of p21 was enhanced in the cell, the rate of cell growth and anchorage-independent growth was inhibited, tumorigenecity was suppressed, the level of expression ofcyclinE andCDK2 decreased while that ofcyclinD1 andCDK4 was not affected. It is suggested that the enhanced expression of p21 markedly inhibits the proliferation and lessens the tumorigenecity of breast cancer cells, and that p21 expression is not related to that ofcyclinD1 andCDK4, but affects the expression ofcyclinE andCDK2.
Databáze: OpenAIRE
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