Expression of heterologous genes inPseudomonas putidaunder the control of streptococcal promoters

Autor:	Frank Laplace, Horst Malke, Jörg Müller, Leo Wollweber
Rok vydání:	1988
Předmět:	Heterologous Promoter Periplasmic space Biology biology.organism_classification Microbiology Pseudomonas putida law.invention Plasmid law Gene expression Genetics Recombinant DNA Molecular Biology Gene
Zdroj:	FEMS Microbiology Letters. 52:275-278
ISSN:	1574-6968 0378-1097
DOI:	10.1111/j.1574-6968.1988.tb02610.x
Popis:	Streptococcal promoters were shown to fulfil primary structure requirements for the expression of heterologous genes in Pseudomonas putida KT2440. The identity of the gene products, streptokinase and human interferon-α1, as synthesized by KT2440, was confirmed by their biological, antigenic and/or physical properties. Although designed as secretion vectors with streptococcal signal sequences, the recombinant plasmids failed to mediate the complete export of either protein into the periplasmic space of KT2440.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::98d958677431511ebffcd9a7e158c9b6 https://doi.org/10.1111/j.1574-6968.1988.tb02610.x Zobrazit plný text záznamu Plný text