Genetic variation in Costa Rican populations of the tropical timber species Cedrela odorata L., assessed using RAPDs

Autor: Carlos Navarro, Jonathan P. Cornelius, Adrian C. Newton, Julia Wilson, A. C. M. Gillies, M. Hernández
Rok vydání: 1997
Předmět:
Zdroj: Molecular Ecology. 6:1133-1145
ISSN: 1365-294X
0962-1083
Popis: Cedrela odorata L. (Spanish cedar), an economically important timber species native to the American tropics, is the focus of increasing conservation concern due to high rates of deforestation within its native range. To assess the extent of the genetic diversity within and between populations of this species, samples were obtained from 10 widely dispersed populations within Costa Rica and analysed for random amplified polymorphic DNA (RAPD) variation. Fourteen 10-mer primers were used to generate 97 polymorphic RAPD bands. Presence/absence data for all bands were subjected to a pairwise genetic distance analysis, according to Jaccard's coefficient, then neighbour-joining cluster analysis was performed on these distances, as was an analysis of molecular variance (AMOVA), to assess levels of differentiation between populations and regions, and Shannon's Diversity Index was used to quantify levels of diversity within and between populations. Results indicated highly significant genetic differentiation (P < 0.004, AMOVA) between populations originating from the North Pacific and Atlantic/South Pacific regions of Costa Rica, with 35.3% of the total variation attributable to a difference between these areas. Little differentiation was recorded between populations from within the same region (P= 0.757, AMOVA), and 65.1% of the total variance was attributable to variation within populations. Estimated values for within-population diversity, calculated as Hpop/Hsp by means of Shannon's Diversity Index, were found to vary greatly between primers, but the overall within-population component of genetic diversity was 0.45. Possible reasons for the high degree of intraspecific genetic variation within this species are discussed and the implications of these results for the conservation and use of its genetic resources are described.
Databáze: OpenAIRE
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