Characteristics of immunofluorescence microscopy and of dilution-plating to detect Pseudomonas syringae pv. phaseolicola in bean seed lots and for risk assessment of field incidence of halo blight

Autor: G. Jochems, A. A. J. M. Franken, J. W. L. Van Vuurde, Y. Birnbaum
Rok vydání: 1991
Předmět:
Zdroj: Netherlands Journal of Plant Pathology. 97:233-244
ISSN: 1573-8469
0028-2944
DOI: 10.1007/bf01989820
Popis: Routine laboratory testing of 710 bean seed lots from various origins forPseudomonas syringae pv. phaseolicola (Psp) with immunofluorescence microscopy (IF) showed that 27.5% of the seed lots (five subsamples of 1000 seeds tested per sample) contained two or more IF-positive cells in a total of 500 microscope fields (magnification 500×). Simultaneously performed dilution-platings of IF-positive subsamples on King's medium B confirmed presence of Psp for one-third of these IF-positive seed lots. The ‘grey area’ of disagreement between both laboratory tests was studied by comparison of test data and by field trials.
Databáze: OpenAIRE