A Charge-Coupled-Device Camera Image Analysis System for Quantifying DNA Distributions in Agarose Gels after Pulsed-Field Gel Electrophoresis

Autor:	D. L. Latz, William C. Dewey, M. L. Trinh, J. F. Ward, L. L. Thompson
Rok vydání:	1994
Předmět:	Gel electrophoresis Radiation Chemistry business.industry Scattering Biophysics Analytical chemistry Charge coupled device camera Fluorescence Electrophoresis chemistry.chemical_compound Optics Personal computer Saturation (graph theory) Agarose Radiology Nuclear Medicine and imaging business
Zdroj:	Radiation Research. 140:37
ISSN:	0033-7587
DOI:	10.2307/3578566
Popis:	A charge-coupled-device camera system was coupled to a personal computer and, with uniformity in illumination and detection (within 4-8%) along each lane, was used for quantifying the distribution of DNA molecules that migrate from the PFGE well (plug) into the lane at distances varying from 1 to 50 mm (with 0.5 mm/pixel). By using a specially designed transmission filter for transmitting 470-725 nm fluorescence from ethidium bromide-stained DNA while eliminating most of the fluorescence (
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9878f25ab4df89d21d3a9d34d659fe16 https://doi.org/10.2307/3578566 Zobrazit plný text záznamu