Purification and Characterization of an Endopolygalacturonase Produced by Sclerotinia Sclerotiorum

Autor:	Mariana Laura Feldman, Mariana Regente, Claudia R. Oliva, L. Dela Canal
Rok vydání:	1999
Předmět:	food.ingredient Chromatography biology Pectin Molecular mass Isoelectric focusing Size-exclusion chromatography Sclerotinia sclerotiorum Ion chromatography Plant Science Horticulture biology.organism_classification chemistry.chemical_compound Isoelectric point food chemistry Ammonium
Zdroj:	Biologia plantarum. 42:609-614
ISSN:	1573-8264 0006-3134
DOI:	10.1023/a:1002691704640
Popis:	An endopolygalacturonase (endo-PG), was purified from the culture medium of a local isolate of Sclerotinia sclerotiorum with ammonium sulphate precipitation, cation exchange chromatography and gel filtration. The purified endo-PG had a molecular mass of approximately 18 kDa estimated by gel filtration. The isoelectric point was determined by isoelectric focusing to be approximately 8, suggesting that PG II possesses a net positive charge at physiological pHs. The pH optimum for the enzyme was at pH 4.5. The endo-PG showed essentially the same affinity for pectin and polygalacturonic acid as substrates.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::98560d0bb512827c812634a979eddc43 https://doi.org/10.1023/a:1002691704640 Zobrazit plný text záznamu Full text from SpringerLink