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Genomic DNA was extracted from blood samples of 91 unrelated Uruguayan males that were initially spotted on FTAR paper (Whatman, Clifton, NJ). The six loci (DYS393, DYS19, DYS389II, DYS390, DYS391, and DYS385) were amplified using the YPLEXTM6 kit (Reliagene Technologies, Inc., New Orleans, LA) according to the manufacturer’s recommendations and to published experiences (1). The amplified products were analysed by capillary electrophoresis using the ABI Prism 310 DNA Sequencer (PE Biosystems, Foster City, CA). Allele assignment was performed by comparison with self-established allelic ladder, positive and negative controls included in the kit. Several external populations were used for comparison from previously published papers; these are samples from Southern Spain and the Spanish Canary Islands (2), Northern Argentinian Amerindians (3), Italians (4), Mozambique (5), Caucasian Argentinians (6), Brazilians (5), and from Portugal (7). Since duplicated loci such as DYS385 cannot be dealt with in AMOVA, the study had to be confined to the systems DYS393, DYS19, DYS389II, DYS390, DYS391. STR allele frequencies, diverse Y-chromosome diversity indices and population differentiation were tested using software ARLEQUIN version 2.000 (8). The allele frequencies and gene diversity of the STR’s DYS393, DYS19, DYS389II, DYS390, DYS391 y DYS385 from Uruguay are shown in Table 1. We counted 76 different haplotypes in a population sample of 91 Uruguayan males giving a haplotype diversity |
