Increased analytical sensitivity of RT-PCR of PSA mRNA decreases diagnostic specificity of detection of prostatic cells in blood
Autor: | Stefan A. Loening, Dietmar Schnorr, Horst Schlechte, Klaus Jung, Birgit Rudolph, Michael Lein, Monika Jung, Christoph Berndt, Wolfgang Henke |
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Rok vydání: | 1997 |
Předmět: |
Cancer Research
medicine.medical_specialty Messenger RNA Pathology business.industry Urology Hyperplasia medicine.disease law.invention Prostate-specific antigen Prostate cancer Real-time polymerase chain reaction medicine.anatomical_structure Oncology Prostate law medicine business Nested polymerase chain reaction Polymerase chain reaction |
Zdroj: | International Journal of Cancer. 70:52-56 |
ISSN: | 1097-0215 0020-7136 |
DOI: | 10.1002/(sici)1097-0215(19970106)70:1<52::aid-ijc8>3.0.co;2-5 |
Popis: | The diagnostic specificity of the detection of disseminated prostatic cells by reverse-transcriptase polymerase chain reaction (RT-PCR) of PSA mRNA was investigated. A sensitive nested PCR was developed. In blood samples from 10 healthy female and 10 healthy male persons examined by RT-PCR, mRNA of PSA was detected 3 times in each group. In the groups of patients suffering from benign prostate hyperplasia and prostate cancer, 6 of 11 and 5 of 12, respectively, gave positive RT-PCR results. With increasing analytical sensitivity of the RT-PCR of PSA mRNA, the diagnostic specificity of the assay is decreased. Further development of this diagnostic method requires the introduction of the quantitative PCR which may make possible discrimination between prostatic and non-prostatic source of PSA mRNA by quantification. |
Databáze: | OpenAIRE |
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