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Recently several reports appeared on the control of glycolysis in living cells, notably tumor cells (Wu, 1964) and cerebral tissues (Lowry et al. 1964). In these studies, the glycolytic intermediates of the cells are assayed enzymatically and the controlling points are analyzed by the “crossover theorem” of Chance et aL(1958). Erythrocytes seemed to be one of the most suitable cells for this kind of analysis, as glycolysis is predominant compared to other metabolic activities. The pattern of glycolytic enzymes are systematically studied by Chapman et al. (1962) and some of the intermediates were assayed chromatographically by Bartlett (1959) and Yoshikawa et aL(l960). Hexokinase has been considered as the rate-limiting step in the glycolytic chain of the cells by several investigators (Rapoport et al.,1961, Chapman et al., 1962, Rose and O’Connell, 1964), as the hexokinase activity is the lowest in the glycolytic enzymes and glycolytic rates of the red cells are found to be parallel to those of hexokinase in several conditions, e.g. aging, storage and in different species of animals. The optimum pH for the glycolysis of the cells lies at pH 8.1, which coincides with those of hexokinase. In this paper, the contents of glycolytic intermediates of human erythrocytes except 1,3diphosphoglycerate are reported, especially in relation to the |
