[26] The RAST principle and the use of mixed-allergen RAST as a screening test for IgE-mediated allergies
| Autor: | T.G. Merrett, J. Merrett |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
Allergy
Chromatography biology Screening test medicine.diagnostic_test Chemistry Radioallergosorbent test Radioimmunoassay Immunoglobulin E medicine.disease medicine.disease_cause respiratory tract diseases Titer Allergen immune system diseases Immunology otorhinolaryngologic diseases biology.protein medicine Antibody |
| Zdroj: | Methods in Enzymology ISBN: 9780121819705 |
| DOI: | 10.1016/s0076-6879(80)70065-4 |
| Popis: | Publisher Summary This chapter describes the radioallergosorbent test (RAST) principle and the preparation of RAST reagents. The chapter discusses how a variant of this test— the mixed RAST technique— can be used as a valuable screening test for atopic allergy. RAST is a solid-phase sandwich RIA. It is a direct assay, eliminates nonspecific serum effects, and measures biologically active allergen-specific IgE sandwiched between allergen and Fc-specific anti-IgE antibodies. In principle, the RAST is similar to the red-cell-linked antigen-antiglobulin reaction (RCLAAR). In RAST test, an excess of insolubilized allergen is incubated with the test serum and binds allergen-specific antibodies, including those of the IgE class. The presence of specific IgE antibodies complexed with allergen is tested, by incubating 125I-labeled, immunosorbent-purified anti-IgE, with the washed complex. The radioactivity associated with the complex is determined after a further washing step and is compared with the results obtained, using reference data. An excess of allergen and labeled anti-IgE is used in the test in order to minimize interference by non-IgE antibodies. The test is semiquantitative because the composition of allergen extracts can vary from batch to batch and test sera can contain IgE antibodies with a range of titers and avidities. |
| Databáze: | OpenAIRE |
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