Kappa and Lambda Determination by Single Colored Immunohistochemistry and Chromogenic in Situ Hybridization Techniques in B-Cell Malignancies
| Autor: | Abeer Attia Saad Eldeen, Reham Mohamed Abo Elregal, Mohamed Tarif Hamza, Dahlia Ahmed Elsewefy, Amany Ahmed Osman |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Pathology
medicine.medical_specialty business.industry Chronic lymphocytic leukemia Chromogenic in situ hybridization medicine.disease Immunoglobulin light chain Lymphoma Lymphoplasmacytic Lymphoma 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine medicine.anatomical_structure immune system diseases hemic and lymphatic diseases 030220 oncology & carcinogenesis medicine Hairy cell leukemia CISH business B cell |
| Zdroj: | The Egyptian Journal of Hospital Medicine. 71:2556-2563 |
| ISSN: | 2090-7125 1687-2002 |
| DOI: | 10.12816/0045656 |
| Popis: | Background: kappa and lambda light chains detection in bone marrow trephine sections help in the determination of B-cell clonality through evaluation of light chain restriction. Aim of the Work: was to compare the efficacy of single color detection-based immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) in evaluating kappa/lambda expression in tissues harboring B-lymphoid lesions. Patients and Methods: Forty patients were enrolled in this study. They were divided into three groups chronic lymphocytic leukemia (CLL/SLL) group I (n=13), non-Hodgkin lymphoma (NHL) group II (n=24) and hairy cell leukemia (HCL) group III (n=3). The 24 NHL cases comprised of (11 diffuse large B-cell lymphoma, 6 mantle cell lymphomas, 3 marginal zone lymphoma, 2 lymphoplasmacytic lymphoma, 1 follicular lymphomas and 1 Burkitt’s lymphoma). Kappa and lambda light chains were detected in their bone marrow trephine sections using single colored immunohistochemistry, chromogenic in situ hybridization and the results were compared to the flowcytometry as reference method. Results: Light chain restriction (LCR) was detected by FCM in 100% of the cases followed by CISH (52.1%; 12/23) of the cases and finally IHC (43%; 18/40). Background: kappa and lambda light chains detection in bone marrow trephine sections help in the determination of B-cell clonality through evaluation of light chain restriction. Aim of the Work: was to compare the efficacy of single color detection-based immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) in evaluating kappa/lambda expression in tissues harboring B-lymphoid lesions. Patients and Methods: Forty patients were enrolled in this study. They were divided into three groups chronic lymphocytic leukemia (CLL/SLL) group I (n=13), non-Hodgkin lymphoma (NHL) group II (n=24) and hairy cell leukemia (HCL) group III (n=3). The 24 NHL cases comprised of (11 diffuse large B-cell lymphoma, 6 mantle cell lymphomas, 3 marginal zone lymphoma, 2 lymphoplasmacytic lymphoma, 1 follicular lymphomas and 1 Burkitt’s lymphoma). Kappa and lambda light chains were detected in their bone marrow trephine sections using single colored immunohistochemistry, chromogenic in situ hybridization and the results were compared to the flowcytometry as reference method. Results: Light chain restriction (LCR) was detected by FCM in 100% of the cases followed by CISH (52.1%; 12/23) of the cases and finally IHC (43%; 18/40). Conclusion: Both conventional CISH and IHC are effective in determining monoclonality in cases of mature B- cell neoplasm that has plasmacytic differentiation and with high amount of cytoplasmic Ig light chains such as MZL and LP. However, they are not effective in determining monoclonality in cases with low amount of Ig light chain such as cases of pregerminal and germinal center lymphoma. Yet, CISH is more informative than IHC due to the lack of background staining which allowed for greater discrimination between absence and presence of monoclonality. |
| Databáze: | OpenAIRE |
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