| Popis: |
The distinction between specific recognition and target cell (TC) lysis has been accepted as a useful concept in the analysis of cytolytic T lymphocyte (CTL)-mediated cytotoxicity. Recently, this process has been resolved by different methods into three steps: binding; “lethal hit”; and TC lysis; the first two of which require direct CTL-TC contact (1,2). As detailed in recent reviews (3–7), CTL-TC conjugates can be isolated physically before the lethal hit step (2,8–10), or binding and lethal hit can be separated on the basis of different cations requirements (11,12), and different susceptibilities to inhibition by low temperature (15®C) and drugs (3). These different sensitivities to temperature, extracellular cations and pharmacological compounds could mean that qualitatively different intracellular activities and/or membrane-associated phenomena operate in the binding and lethal hit steps. |
