Dynamic Structures of DNA Heptamers with Different Central Trinucleotide Sequences Studied by Electrochemical and Spectral Methods

Autor:	Michaela Vorlíčková, Libuše Trnková, Iva Kejnovská, Iveta Pilarova
Rok vydání:	2014
Předmět:	0303 health sciences Circular dichroism Chemistry Supramolecular chemistry Analytical chemistry macromolecular substances 010402 general chemistry Electrochemistry 01 natural sciences 0104 chemical sciences Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound Crystallography Duplex (building) Molecule Voltammetry Polyacrylamide gel electrophoresis DNA 030304 developmental biology
Zdroj:	Electroanalysis. 26:2118-2128
ISSN:	1040-0397
Popis:	A short, single-stranded DNA fragment d(GCGAAGC) and its analogs d(GCNNNGC) carrying different trinucleotide sequences in the center of the molecule (AAA,CCC, GGG or TTT) were investigated by spectral (CD and UV spectra) and electrochemical methods (voltammetry, polyacrylamide gel electrophoresis). Our results show that (i) the central triplet GAA or AAA dramatically stabilizes DNA hairpins, (ii) a stem-loop configuration is not supported by CCC or TTT sequences, instead, these heptamers adopt bimolecular duplex forms. Finally (iii), in the case of GGG sequence a very stable supramolecular G-quadruplex is formed.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::98128914066774d02db4c3b2977cadfe https://doi.org/10.1002/elan.201400288 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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