Antifreeze glycoproteins in the plasma of Newfoundland Atlantic cod (Gadus morhua)

Autor:	Choy L. Hew, Shashikant B. Joshi, Garth L. Fletcher, Don Slaughter
Rok vydání:	1981
Předmět:	Biochemistry biology Antifreeze protein Gadus Animal Science and Zoology Antifreeze Glycoproteins biology.organism_classification Atlantic cod Ecology Evolution Behavior and Systematics
Zdroj:	Canadian Journal of Zoology. 59:2186-2192
ISSN:	1480-3283 0008-4301
Popis:	The plasma of the Atlantic cod, Gadus morhua, contained antifreeze glycoproteins which were present only during the winter months. The antifreeze proteins were isolated, using gel filtration and ion exchange chromatography, and characterized by high performance liquid chromatography. The antifreeze proteins appeared to consist of at least seven components with molecular weights ranging from 2 500 to 33 000. Chemical analysis of the larger components showed a predominance of alanine, threonine, and galactosamine in a ratio of 2:1:1. The smaller peptides contained proline, in addition to alanine and threonine. The amino acid sequence of the smallest glycopeptide (molecular weight 2500) was found to be Ala Ala Thr Pro Ala Thr Ala Ala Thr Pro Ala Thr Ala Ala.These glycoproteins are very similar, if not identical, in amino acid and carbohydrate composition to those isolated from Antaractic nototheniids and several northern gadoids. The sequence of the smallest glycopeptide from the Atlantic cod is identical to that reported for the polar cod, Boreogadus saida.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_________::9806a98428523af24f0e270db9aedc72 https://doi.org/10.1139/z81-296 Zobrazit plný text záznamu