| Popis: |
Whether sandwich enzymeimmunoassay (EIA) could be used for the determination of x-carrageenan (x-CA) or not was studied. Furthermore, EIA was compared with double diffusion method and quantitative precipitation reaction. Antisera were obtained from rabbit and guinea pig immunized with x-CA, methylated bovine serum albumin and complete Freund's adjuvant. In EIA, the anti-x-CA rabbit serum as a capture antibody on the solid phase and anti-x-CA guinea pig serum as a detector antibody were used. Further, peroxidase-conjugated anti-guinea pig IgG rabbit antibody and a combination of hydrogen peroxide and orthophenylenediamine were used for color development. In EIA, most suitable dilutions for the determination of x-CA were 20000-fold in both rabbit and guinea pig antisera. The range of determination were from 64 to 512 ng/ml. Cross reaction with some additives with similar uses to x-CA was not observed except that agar slightly showed the reaction. EIA was superior in the sensitivity, the range of determination and the amount of antiserum required to two other methods, double diffusion method and quantitative precipitation reaction. Thus, it was suggested that EIA may be a useful method for the determination of x-CA. |
