miRNA-mediated expression switch of cell adhesion genes driven by microcirculation in chip
| Autor: | Udo Schumacher, Dmitry A. Sakharov, Maxim Shkurnikov, Timur R. Samatov, Alexander G. Tonevitsky, N. V. Senyavina, S. A. Tonevitskaya, Vladimir V. Galatenko, Alexey V. Galatenko, Hermann Ehrlich, Uwe Marx |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
L1 biology Microarray analysis techniques CD44 Biomedical Engineering Cancer Bioengineering medicine.disease Cell biology 03 medical and health sciences 030104 developmental biology 0302 clinical medicine 030220 oncology & carcinogenesis CYR61 microRNA biology.protein medicine Electrical and Electronic Engineering Cell adhesion Gene Biotechnology |
| Zdroj: | BioChip Journal. 11:262-269 |
| ISSN: | 2092-7843 1976-0280 |
| Popis: | Changes in cell adhesion molecule (CAM) expression and miRNAs regulating them are known to be involved in malignant progression in colon cancer. We investigated expression profiles of CAM genes and non-coding RNAs in CaCo2 colon cancer cells in static culture and under dynamic flow conditions perfused in microfluidic chip emulating physiological microenvironment. We incubated monolayers of CaCo2 cells in Transwell® units either under static conditions or under flow in a microfluidic chip. We identified 7 up-regulated CAM genes (CD44, CDH7, CEACAM5, CEACAM6, CYR61, L1CAM and VCAN), 7 down-regulated genes (COL12A1, FGA, FGB, FGG, GJA1, ITGA5 and LAMA1) and 69 miRNAs targeting them under the influence of microcirculation. The revealed network comprised CAM genes known to interact with each other and 13 miRNAs simultaneously regulating more than one of them. The discovered regulatory network comprising CAM genes and miRNAs is likely involved in normal functioning of intestine epithelium as well as in cancer progression. |
| Databáze: | OpenAIRE |
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