Molecular characterization and antibacterial activity of oral antibiotics and copper nanoparticles against endodontic pathogens commonly related to health care-associated infections
| Autor: | Helia Bello-Toledo, Gerardo González-Rocha, Fernanda Katherine Sacoto-Figueroa, Celia A. Lima, Gabriela Sánchez-Sanhueza, Luis Luengo Machuca, Manuel Melendrez-Castro |
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| Rok vydání: | 2021 |
| Předmět: |
Minimum bactericidal concentration
biology Tetracycline medicine.drug_class Pseudomonas Antibiotics Erythromycin 030206 dentistry biology.organism_classification Antimicrobial Enterococcus faecalis Microbiology 03 medical and health sciences Minimum inhibitory concentration 0302 clinical medicine 030220 oncology & carcinogenesis medicine General Dentistry medicine.drug |
| Zdroj: | Clinical Oral Investigations. 25:6729-6741 |
| ISSN: | 1436-3771 1432-6981 |
| Popis: | To carry out molecular characterization and determine the antibacterial activity of oral antibiotics and copper nanoparticles (Cu-NPs) against endodontic strains isolated from persistent infections. Root canal samples from 24 teeth in different patients with persistent endodontic infections were obtained. The isolated strains were identified by biochemical tests and 16S rDNA sequencing. Genotyping was achieved by molecular methods. The antibacterial activity of antibiotics and copper nanostructures was determined by using minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values. Furthermore, a time-kill kinetics assay was evaluated. Nonparametric tests (Kruskal-Wallis ANOVA) were performed (p value |
| Databáze: | OpenAIRE |
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