Non-labeled selective virus detection with novel SERS-active porous silver nanofilms fabricated by Electron Beam Physical Vapor Deposition
| Autor: | Rustam R. Guliev, Andrey K. Sarychev, Andrey N. Lagarkov, Artem P. Tkachuk, Sergey L. Kalnov, Aleksei N. Mukhin, I. A. Boginskaya, E. V. Putlyaev, Ilya A. Rodionov, Marina V. Balandina, Vladimir A. Gushchin, Aidar R. Gabidullin, A. V. Eremenko, Ilya A. Ryzhikov, E.A. Trifonova, Nikolay N. Durmanov, Ilya N. Kurochkin |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Materials science viruses Nanotechnology 02 engineering and technology Electron beam physical vapor deposition Virus 03 medical and health sciences symbols.namesake Materials Chemistry Tobacco mosaic virus Electrical and Electronic Engineering Instrumentation Nanoscopic scale Metals and Alloys Substrate (chemistry) 021001 nanoscience & nanotechnology Condensed Matter Physics Surfaces Coatings and Films Electronic Optical and Magnetic Materials 030104 developmental biology Chemical engineering Physical vapor deposition symbols 0210 nano-technology Raman spectroscopy Biosensor |
| Zdroj: | Sensors and Actuators B: Chemical. 257:37-47 |
| ISSN: | 0925-4005 |
| Popis: | Virus detection is often performed using antibody-based and polymerase chain reaction-based techniques. Such methods have major deficiencies, caused by time-consuming and labor-intensive incubation and purification steps. In this contribution, a novel SERS substrate for qualitative virus detection was developed and described. The substrate is composed of a thin silver film with folded surface structure containing pore-like nanoscale cavities and indentations, deposited on mica substrate by electron beam physical vapor deposition method. Pore-like structures are semi-regularly arrayed, with a rough surface in between, allowing for SERS activity, and their size and periodicity can be manipulated in the manufacturing process. It was speculated that viral particles could be trapped in these structures and would generate easily detectable enhanced Raman signals. The SERS substrate was tested against detection of four virus species – rabbit myxomatosis virus, canine distemper virus, tobacco mosaic virus and potato virus X. Specific spectra were obtained and analyzed for each virus. Data analysis demonstrated successful differentiation between tested species. The reported results demonstrate that novel SERS substrate is suitable for detection and identification of viral particles. |
| Databáze: | OpenAIRE |
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