Novel fluorescently labeled nucleotides: synthesis, spectral properties and application in polymerase chain reaction
| Autor: | Alexander S. Zasedatelev, Sergey A. Lapa, Evgeniy E. Fesenko, V. E. Kuznetsova, Tatyana V. Nasedkina, V. E. Shershov, T. O. Guseinov, M. A. Spitsyn, Mariya A. Avdonina, Alexander V. Chudinov, Anna Yu. Ikonnikova |
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| Rok vydání: | 2016 |
| Předmět: |
chemistry.chemical_classification
biology 010405 organic chemistry Chemistry Spectral properties General Chemistry 010402 general chemistry 01 natural sciences 0104 chemical sciences law.invention chemistry.chemical_compound Real-time polymerase chain reaction Biochemistry law biology.protein Nucleotide DNA Polymerase chain reaction Polymerase Taq DNA Polymerase |
| Zdroj: | Mendeleev Communications. 26:95-98 |
| ISSN: | 0959-9436 |
| Popis: | The novel C5-modified nucleotides containing indodicarbocyanine dye bearing a carboxyl group at the 3-position of indolenine have been synthesized and incorporated into DNA by commercially available thermostable Taq DNA polymerase. The efficiency of incorporation of labeled nucleotides during polymerase chain reaction was studied in a TB-biochip and THROMBO-Biochip test systems. |
| Databáze: | OpenAIRE |
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