Purified winged bean protease inhibitor affects the growth ofBactrocera cucurbitae
| Autor: | S K Sohal, A P Kaur |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
Psophocarpus Chymotrypsin Protease biology medicine.medical_treatment General Medicine biology.organism_classification Trypsin 010603 evolutionary biology 01 natural sciences Protease inhibitor (biology) 010602 entomology Horticulture Insect Science medicine biology.protein Instar Bactrocera PEST analysis Agronomy and Crop Science medicine.drug |
| Zdroj: | Bulletin of Entomological Research. 109:550-558 |
| ISSN: | 1475-2670 0007-4853 |
| DOI: | 10.1017/s0007485318000913 |
| Popis: | The melon fruit fly,Bactrocera cucurbitae(Coquillett), is a serious agricultural pest which has defied the various control measures employed against it. Protease inhibitors present in plants which have the potential to inhibit the growth and development of associated insect pests can be a possible alternative which can be manipulated for developing resistance in plants to the pest. In the present study, winged bean (Psophocarpus tetragonolobus) protease inhibitor isolated through affinity chromatography was explored for its potential to disrupt the development of melon fruit fly,B. cucurbitae. Different concentrations (12.5, 25, 50, 100, 200, and 400 µg ml−1) of the winged bean protease inhibitor (WBPI) were incorporated into the artificial diet of the second instar (64–72 h old) larvae ofB. cucurbitae. The WBPI significantly delayed the larval, pupal, and total development period. The percentage pupation and adult emergence of the treated larvae was reduced as compared with control. The activities of major digestive enzymes (trypsin, chymotrypsin, leucine aminopeptidase, and elastase) decreased significantly in the larvae treated with different concentrations (50, 100, 200, and 400 µg ml−1) of WBPI. The findings reveal that the inhibitor holds considerable promise for the management of the melon fruit fly. |
| Databáze: | OpenAIRE |
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