Reduction of Sample Matrix Effects - The Analysis of Benzimidazole Residues in Serum by Immunobiosensor

Autor:	G. Andrew Baxter, Lars Johnsson, Christopher T. Elliott, David L. Brandon, Steven R. H. Crooks
Rok vydání:	2002
Předmět:	Detection limit Benzimidazole Residue (complex analysis) Chromatography Chemistry Immunology Analytical technique Assay sensitivity chemistry.chemical_compound Surface plasmon resonance Agronomy and Crop Science Quantitative analysis (chemistry) Biosensor Food Science
Zdroj:	Food and Agricultural Immunology. 14:209-216
ISSN:	1465-3443 0954-0105
DOI:	10.1080/09540100220145000a
Popis:	Regulatory authorities, the food industry and the consumer demand reliable determination of chemical contaminants present in foods. A relatively new analytical technique that addresses this need is an immunobiosensor based on surface plasmon resonance (SPR) measurements. Although a range of tests have been developed to measure residues in milk, meat, animal bile and honey, a considerable problem has been encountered with both serum and plasma samples. The high degree of non-specific binding of some sample components can lead to loss of assay robustness, increased rates of false positives and general loss of assay sensitivity. In this paper we describe a straightforward precipitation technique to remove interfering substances from serum samples to be analysed for veterinary anthelmintics by SPR. This technique enabled development of an assay to detect a wide range of benzimidazole residues in serum samples by immunobiosensor. The limit of quantification was below 5 ng/ml and coefficients of variation were ...
Databáze:	OpenAIRE
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