Growth kinetics of 2- and 3-D cell models as influenced by the microenvironment

Autor: Ostapchenko Li, L. V. Garmanchuk, M. V. Sidorenko, E. M. Perepelitsina
Rok vydání: 2009
Předmět:
Zdroj: Cytology and Genetics. 43:305-310
ISSN: 1934-9440
0095-4527
Popis: The noncontact cocultivation system was developed for the study of the paracrine interactions between MCF-7 (breast carcinoma cells) and MT-4 (a line of human T-cell leukemia). Viability and proliferation rates were determined in the adhesion and suspension fractions of MCF-7 cells sampled from two model systems: monolayer culture and multicellular tumor spheroids (MTS). Cocultivation with MT-4 reduced the number of MCF-7 cells in the adhesion fraction and had no effect upon the suspension fraction, despite an increase in the total population of MCF-7 cells. The two model systems displayed a substantial difference in cell viability, alone and in the presence of MT-4 cells — the fraction of viable cells in the monolayers was greater than in the spheroids. It is suggested that cocultivation with MT-4 stimulates proliferation of MCF-7 cells via a paracrine mechanism, reduces adhesion to the substrate, and leads to MTS formation.
Databáze: OpenAIRE
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