| Popis: |
In this work was optimized the composition of fermentation media for the growth of Phoma sp., which is a potential fungi to be used for the production of bioherbicide. For this purpose, the fungal growth was optimized, firstly, using synthetic media containing glucose, peptone and yeast extract. In a second moment, glucose was changed by sucrose food-grade and peptone and yeast extract by corn steep liquor and the growth optimized again. The optimized composition of synthetic media was glucose and peptone 20 g.L -1 , yeast extract 7.5 g.L -1 and initial pH of 6.0. For industrial media, the optimized composition was sucrose 20 g.L -1 , CSL 8% and initial pH of 6.0. At these conditions, maximum fungal biomass were 22 and 33 g.L -1 , respectively. This study shows that industrial medium was better than synthetic medium and provides cost savings for a possible increase in production scale. |
