| Popis: |
Adipose derived stromal vascular fraction (SVF) contains a heterogeneous population of mononuclear cells, progenitor cells and about 1-10% are mesenchymal stromal cells. These cells are an ideal candidate for regenerative medicine for peripheral neuropathy. Leprosy is a disabling disorder with neuropathy, usually with consequences of permanent disability of the extremities. Before we could inject the SVF, we need to learn whether the SVF is appropriate to be used for neuropathy treatment using the flow cytometry characterization. Liposuction was done to four leprosy patients and adipose tissue was processed into SVF with a closed system. Following the process, the SVF were characterized and counted the CD73, CD90, CD34, CD45, Lin-Negatif, and CD105 cells using flow cytometry, cell counting, sterility and presence of mycobacteria. The process was done by ProStem using a traditional flow cytometer, without blocking and negative isotypes. The expected results from these protocols are the appropriate amount of mesenchymal cells needed to meet the IFATS and ISCT standards for SVF. |
