Hydrophilic interaction chromatography as an alternative to reversed-phase HPLC in determining anthocyanins and betacyanins
| Autor: | Victor I. Deineka, I. I. Saenko, L. A. Deineka, I. P. Blinova |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Chromatography
Glycosylation Chemistry Hydrophilic interaction chromatography 010401 analytical chemistry Diol food and beverages Reversed-phase chromatography 010402 general chemistry 01 natural sciences 0104 chemical sciences Analytical Chemistry carbohydrates (lipids) chemistry.chemical_compound Stationary phase Betacyanins Organic chemistry |
| Zdroj: | Journal of Analytical Chemistry. 71:297-301 |
| ISSN: | 1608-3199 1061-9348 |
| DOI: | 10.1134/s1061934816030035 |
| Popis: | We selected the conditions of hydrophilic interaction chromatography for separating anthocyanins and betacyanins using a diol stationary phase and mobile phases of acetonitrile–0.5 vol % phosphoric acid–water. Regularities of the separation of betacyanins and cyanidine derivatives of various structures are studied by analyzing their relative retentions. It is shown that the approach is efficient for the preliminary classification of anthocyanins by the type of glycosylation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|